DISSERTAÇÃO DE MESTRADO #769 – JOÃO RENATO VAZ PIRES – 17/07/2025

Defocusing Microscopy (DM) is a quantitative phase contrast microscopy technique based on the propagation of the electrical field through the microscope, which is used to obtain morphological and biomechanical parameters of different cells. On this dissertation, a geometrical model of the surface of a red blood cell was used to make a proof of concept for the total 3D reconstruction using DM. Furthermore, a new model for the angular spectrum of a phase object was studied and compared with the one used in previous works. Finally we were able to make a computational routine to achieve the total 3D reconstruction of red blood cells in mediums with different osmolalities. Using this new routine, the results were obtained in 0.4s with high accuracy, representing an improvement from previous results, and enabling the total 3D reconstruction in real time.

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