Colóquio Extra 10/11/2023: Nucleic acid analogues as tools for research and biomedical applications
- Palestrantes: Kira Astakhova - Technical University of Denmark
Sobre este evento
The molecular structure of nucleic acid monomers encodes their interactions and hence, biological properties. Synthetic analogues allow for deeper understanding of natural structural and interaction aspects of nucleic acids and provide diagnostic and therapeutic tools.
In this talk our most recent studies on nucleic acid analogues will be presented. First, hydrophobic polyaromatic hydrocarbon modifications will be presented as a tool to detect human short tandem repeats (STR). STRs are short and include many stop codons making them difficult for detection with polymerases. We solve this issue using polymerase-free solid-support detection with synthetic oligonucleotide probes including modifications that enhance binding affinity and specificity to STR and provide with fluorescence response.
Second, locked nucleic acids (LNA) will be presented as a highly effective nucleic acid analogue for detecting cancerogenic mutations in human regulatory microRNA.
Last, interaction of nucleic acids with components of immune system will be discussed. Our most recent work on targeting antibodies with nucleic acid aptamers will be presented.
References:
https://pubs.acs.org/doi/10.1021/acs.analchem.3c00063
Mutations in microRNA-128-2-3p identified with amplification-free hybridization assay | PLOS ONE
| Mutations in microRNA-128-2-3p identified with amplification-free hybridization assay
We describe a quantitative detection method for mutated microRNA in human plasma samples. Specific oligonucleotides designed from a Peyrard-Bishop model allowed accurate prediction of target:probe recognition affinity and specificity. Our amplification-free tandem bead-based hybridization assay had limit of detection of 2.2 pM. Thereby, the assay allowed identification of single-nucleotide polymorphism mismatch profiles in clinically relevant microRNA-128-2-3p, showing terminal mutations that correlate positively with inflammatory colitis and colorectal cancer. |
Scaffolding along nucleic acid duplexes using 2′-amino-locked nucleic acids – PubMed (nih.gov)
____________________________________________________________________________
Sobre a palestrante
Msc degree in organic chemistry 2006 IBCH Moscow, Russia, PhD in chemistry 2009, IBCH and Nucleic research center, SDU, Denmark. Postdoc at SDU, UCSB and Stanford, USA; from 2012 run a research group focusing on chemistry and biology of nucleic acids. From 2017 joined Technical University of Denmark. Have 4 spinouts. Chairwoman of the Danish Chemical Society and representative of Denmark in European Chemical Society.
_____________________________________________________________________________
Para assistir remotamente via Zoom,
Entrar Zoom Reunião
https://us06web.zoom.us/j/81434558771?pwd=TuFDMCLaix8I4Ppp06aCbXavUcYgsD.1
ID da reunião: 814 3455 8771
Senha: 801561
Lembramos que essa palestra será gravada.